rherbb3 fc (R&D Systems)

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R&D Systems rherbb3 fc
Rherbb3 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+erbb3+fc+chimera/Recombinant+Human+ErbB3%2FHer3+Fc+Chimera+Protein%2C+CF/us12486332-475-19-20
Average 93 stars, based on 16 article reviews

rherbb3 fc - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Direct Binding of the EGF-like Domain of Neuregulin-1 to Integrins (?v?3 and ?6?4) Is Involved in Neuregulin-1/ErbB Signaling
Article Snippet: .. Recombinant human ErbB3 Fc chimera (R&D Systems) was immobilized to wells of 96-well microtiter plates at 1 μg/ml coating concentration in 0.1 m NaHCO 3 , pH 9.4, overnight at 4 °C, and the remaining protein-binding sites were blocked by incubating with 0.1% BSA. ..

Article Title: Direct Binding of the EGF-like Domain of Neuregulin-1 to Integrins (αvβ3 and α6β4) Is Involved in Neuregulin-1/ErbB Signaling
Article Snippet: .. Recombinant human ErbB3 Fc chimera (R&D Systems) was immobilized to wells of 96-well microtiter plates at 1 g/ml coating concentration in 0.1 M NaHCO3, pH 9.4, overnight at 4 °C, and the remaining OCTOBER 8, 2010 • VOLUME 285 • NUMBER 41 JOURNAL OF BIOLOGICAL CHEMISTRY 31389 by guest on M ay 9, 2015 http://w w w .jbc.org/ D ow nloaded from protein-binding sites were blocked by incubating with 0.1% BSA. .. Wells were then incubated with biotinylated GST fusion NRG1 in the presence of non-labeled GST, GST-WTNRG1, or incubating with protein A-Sepharose (GEHealthcare) for 1 h at 4 °C and washed 3 times with wash buffer (20 mM HEPES, pH 7.4, 100 mM NaCl, 10% glycerol, 0.5% Nonidet P-40, 1 mM 31390 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 285 • NUMBER 41 • OCTOBER 8, 2010 MgCl2, 1 mM phenylmethylsulfonyl fluoride, 20 mMNaF, 1 mM Na3VO4, protease inhibitor mixture (Sigma).

Article Title: Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3.
Article Snippet: During the past 15 years, antibodies have been established as the primary affinity proteins used in cancer therapy [1, 2].. More recently, promising alternative scaffolds of both immunoglobulin and non-immunoglobulin origins have gained increased interest [3, 4].. Several of these alternative molecular formats demonstrate an improved stability and solubility, more cost-efficient production, and ease of manipulation, as compared to antibodies [3–5]. antibodies, as well as other large proteins, have limited vascular permeability and tumor-penetrating capabilities [6]. .. During the past 15 years, antibodies have been established as the primary affinity proteins used in cancer therapy [1, 2].. More recently, promising alternative scaffolds of both immunoglobulin and non-immunoglobulin origins have gained increased interest [3, 4].. Several of these alternative molecular formats demonstrate an improved stability and solubility, more cost-efficient production, and ease of manipulation, as compared to antibodies [3–5]. antibodies, as well as other large proteins, have limited vascular permeability and tumor-penetrating capabilities [6]. ..

Article Title: Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3
Article Snippet: Binding kinetics for ErbB3-binding ABD variants to albumin and ErbB3 were evaluated by SPR using a ProteOn XPR36 protein interaction array system (Bio-Rad, Hercules, CA, USA) and compared to non-randomized ABD. .. Recombinant human ErbB3–Fc chimera (R&D Systems), His 6 -tagged human ErbB3 (Sino Biological, Beijing, China), His 6 -tagged murine ErbB3 (mErbB3; Sino Biological), human serum albumin and murine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) were immobilized to 2,000–4,000 RU in separate flow cells of general layer medium (GLM) sensor chips using standard amine coupling according to the manufacturer’s recommendations. ..

Article Title: Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3.
Article Snippet: Analysis of binding kinetics Binding kinetics for ErbB3-binding ABD variants to albumin and ErbB3 were evaluated by SPR using a ProteOn XPR36 protein interaction array system (Bio-Rad, Hercules, CA, USA) and compared to non-randomized ABD. .. Recombinant human ErbB3–Fc chimera (R&D Systems), His6-tagged human ErbB3 (Sino Biological, Beijing, China), His6-tagged murine ErbB3 (mErbB3; Sino Biological), human serum albumin and murine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) were immobilized to 2,000–4,000 RU in separate flow cells of general layer medium (GLM) sensor chips using standard amine coupling according to the manufacturer’s recommendations. ..

Article Title: Direct Binding of the EGF-like Domain of Neuregulin-1 to Integrins (αvβ3 and α6β4) Is Involved in Neuregulin-1/ErbB Signaling
Article Snippet: Horseradish peroxidase-conjugated anti-rabbit IgG was purchased from Bio-Rad. .. We obtained recombinant human NRG1 EGF-like domain peptide (residues Ser-177— Lys-241, synthesized in Escherichia coli, 97% purity), recombinant human NRG1 isoform SMDF (296 amino acids, Spodoptera frugiperda Sf21(baculovirus)-derived), and recombinant human ErbB3 Fc chimera from R&D Systems (Minneapolis, MN). ..

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